Bullet CBB Stain Lite
Bullet CBB Stain Lite is a one-step, 15 minutes complete Coomassie Brilliant Blue staining solution. Compared to our previous
product, it reduces staining time and steps, allowing for a clear staining image in a shorter period. Additionally, since there is
no need for a destaining step, the staining image can be observed during the staining process.
Features
- In one step, complete staining in 15 minutes
No need for gel pre-staining washing
Clear staining image without the need for a destaining step
- Free from strong irritants like acetic acid and methanol
Applications
Staining image within the staining solution
Stained protein samples after electrophoresis using this product. User can visualize staining image within a tray containing the staining solution using this product.
Conditions
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| Sample | Protein extract from E. coli,dissolve in sample buffer (for SDS-PAGE, 2x, 2-ME) (#30566-22) |
|---|---|
|
SDS-PAGE |
Bullet PAGE Plus Precast Gel, 5-20%, 17wells(#21794-54）400V(constant voltage), for 10 minutes |
|
CBB stain |
Bullet CBB Stain Lite(#21964）at room temperature for 15 minutes |
Comparison
Comparison of various CBB staining solutions
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| Bullet CBB Stain Lite （#21964） |
CBB Stain One Super （#11642） |
CBB Stain One （#04543） |
Rapid Stain CBB Kit （#30035） |
|
|---|---|---|---|---|
| Dye | CBB G-250 | CBB R-250 | CBB G-250 | CBB R-250 |
| Package contents |
|
|
|
|
| Pretreatment | No need | Washing (3 times x 5 mins.) |
Washing (3 times x 5 mins.) |
Adjustment （Mixing two liquids） |
| Staining time | 15 mins.* | 30 mins. | 60 mins. | 20 mins. |
| Destaining | No need* | Over 1 hr. | ||
| Detection sensitivity | Several tens of ng (BSA) | |||
＊ For gel drying/preservation, stain and destain for 1 hour each.
Example of staining
Staining image after staining procedure (without destaining)
This product allows user to visualize staining image with a low background without destaining step.
Staining image after destaining step
This product allows for the visualization of staining image after 15 minutes of staining without a destaining step. However, by extending both staining and destaining steps to 60 minutes each, a clearer staining image can be achieved.
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| Sample | Diluted Protein Markers (10x)(#29458-24) into ① 3x, ② 1x, ③ 1/3x, and ④ 1/10x, then added 2 µL. |
|---|---|
|
SDS-PAGE |
Extra PAGE One Precast Gel, 5-20%, 13wells（#13063-74） 400 V（constant voltage）, for 30 mins. |
Ordering information
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| Product name | Storage | Product number | Package size | Price |
|---|---|---|---|---|
| Bullet CBB Stain Lite | Refrigerate | 21964-24 | 50 mL |  |
| 21964-95 | 500 mL |
